Availability: Please contact us to check the availability
Immunogen: Purified Antigen from Thymocyte and T-cell extract
UniProt number: PGo to UniProt/SwissProt173Go to UniProt/SwissProt (CD4_HUMAN)
Product presentation: Cell culture medium with phosphate buffer, BSA and 0.09 % sodium azide**.
Recommended secondary reagent: We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
About: Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Pretreatment: The antigen epitope detected by clone MEM-115 may be sensitive to the treatment with H2O2. Therefore it is recommended to block endogenous peroxidases with 0.5% H2O2 in Methanol (10 min incubation). This step should be carried out prior to antigen unmasking! Antigen unmasking is performed at 96-100° Celsius in 1 mM EDTA pH 8.0 for 10-20 min.
Description: This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
Properties: Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Tips: *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Test: bio logo supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Litterature: 1. Falini B, Stein H, Pileri S, Canino S, Farabbi R, Martelli MF, Grignani F, Fagioli M, Minelli O, Ciani C, et al. Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies. Histopathology. 1987 Dec;11(12):1229-42. 2. Stein H, Spiegel H, Herbst H, Niedobitek G, Foss HD. [Lymphoid tissues and AIDS: role of lymphocytes and follicular dendritic cells(FDC)]Verh Dtsch Ges Pathol. 1991;75:4-19. Review. German. 3. Spiegel H, Herbst H, Niedobitek G, Foss HD, Stein H. Follicular dendritic cells are a major reservoir for human immunodeficiency virus type 1 in lymphoid tissues facilitating infection of CD4+ T-helper cells. Am J Pathol. 1992 Jan;140(1):15-22. 4. Daum S, Weiss D, Hummel M, Ullrich R, Heise W, Stein H, Riecken EO, Foss HD; Intestinal Lymphoma Study Group. Frequency of clonal intraepithelial T lymphocyte proliferations in enteropathy-type intestinal T cell lymphoma, coeliac disease, and refractory sprue. Gut. 2001 Dec;49(6):804-12.